megablast - Sigenae 8 on cg_v0.1.2
options -G 1 -E 1
http://aquacul4.fish.washington.edu/~steven/filefish/megablast_sig8_v012.out


blastn - Sigenae 8 on cg_v0.1.2 default
http://aquacul4.fish.washington.edu/~steven/filefish/blastn_sig8_v012.out



converting blastn to gff
step1
make blast more accurate…

original ~11 million rows..

c3>=95


~ 2million rows

evalue < 1e-20

~140k

algn length >100

~100k

Galaxy79-[Filter_on_data_78].tabular

Converting to gff

old code
./Blast2Gff.pl -i /Volumes/Bay3\ scratch/gff_fun/7 -o /Volumes/Bay3\ scratch/gff_fun/Combined_fosmids_cd_hit_mod_20000_7trim.gff -d "sigenae_v8" -p EXON -s "something"

./2_Blast2Gff.pl -i /Volumes/Bay3\ scratch/gff_fun/Sig8blastv012 -o /Volumes/Bay3\ scratch/gff_fun/cgigas_v012_1.gff -d "sigenae_v8" -p EXON -s "something"






./2_Blast2Gff.pl -i /Volumes/Bay3\ scratch/gff_fun/sigena8_blast_v010trimmed3.txt -o /Volumes/Bay3\ scratch/gff_fun/sig8_blast_v010_F.gff -d "sigenae_v8" -p EXON -s "something"